2. Average reaction rate data from the Energy and Enzymes lab
3. Graph and analyze this data
H.W. Study for tomorrow's CH.2 TEST AND put together your Ch. 2 Unit Packet (this includes the Ch. 2 Super Review)- Click on the link to open and print or copy the table of contents. Points will be deducted if this is missing.
AND Finish the Energy and Enzymes Lab:
–Pre
lab questions
–Hypotheses
–Procedure
pictures with the completed summary of test tube contents table.
–Completed
data table
–Graph:
Graph your data: Create 1 graph
for your data using graph paper.
Obtain the class averages for the rate of
reaction for your period. USING GRAPH
PAPER, graph the class average reaction
rate (change in temperature per second) data for Test Tubes #1-#6. This should be a bar graph. Graph the reaction rate as your y-axis and
test tube contents as your x-axis. When
labeling your test tube contents on the x-axis be specific in what the contents
were. For ex. 5 mL H2O2,,
Raw Liver + 5 mL H2O2,etc… be specific! DO NOT just
label the contents test tubes #1, #2… Be
sure to include a title, axis labels and a key (if necessary). Identify the independent variables and the
dependent variables.
–Analysis
questions:
Analysis: On your separate paper, under the data table, write the heading ANALYSIS. Then answer the questions below in complete sentences.
Analysis: On your separate paper, under the data table, write the heading ANALYSIS. Then answer the questions below in complete sentences.
1. Describe at least two things that you observed that would indicate a
chemical reaction occurring. Which liver
sample had the most vigorous chemical reaction? Was this reaction endothermic
or exothermic ; support this answer with data you gathered from the lab?
2. In your graph, you compared the reaction rate of hydrogen peroxide
breakdown without an enzyme to the reaction rate of hydrogen peroxide breakdown
in the presence of the enzyme catalase.
What conclusions can you draw from the graph of your data? How do the rates of these two test tubes
compare (see calculation in data table)?
3. In your graph, you used data from catalase at room temperature, in
cold water, and catalase that had been cooked.
a. Use the data from the lab to describe which temperature was the
closest to catalase’s optimal reaction temperature.
b. Using
vocabulary that you learned in class, describe what happened to the catalase in
the cooked liver. What effect did this
have on the rate of the reaction in Test Tube #4?
c. How did the cool temperature impact the rate
of reaction for catalase? Justify your
answer using data from the lab and your knowledge of what happens to molecules
as they cool down.
4. In your graph, you used data from catalase in water and catalase in
vinegar. How did vinegar affect the
catalase? Justify your answer using data
from your lab/graph and your knowledge of pH and proteins from class.
5. In your graph, you used data from 5mL of hydrogen peroxide exposed to
catalase as compared to 10mL of hydrogen peroxide exposed to catalase. What did you change in this part of the
lab? What effect did this change have on
the reaction rate? Justify your answer using
data from your lab/graph and information that you’ve learned in class.
6. Using page 52 in your book to help you, draw a series of pictures to
show how catalase binds to hydrogen peroxide to create water and oxygen as
products. Label enzyme, catalase, active site, substrate, products, hydrogen
peroxide, water, and oxygen in the illustration. Make sure that the picture represents the
catabolic reaction that occurred in this lab.
7. Draw another picture that shows
catalase at room temperature and another
picture that shows catalase after exposure to high heat (aka boiling) or acidic
pH.
