1. W.U. A Nobel for Illuminating Biology (article and questions)
2. Transformation Lab: the exciting results!!!! (absent today? Make sure you get your lab results from another member of your lab group.)
H.W. Start typing your pGlo Transformation formal lab report
DUE MONDAY NIGHT AT 11:59 PM TO TURNITIN.COM AND A HARD COPY IS DUE TO ME ON TUESDAY
Analysis Questions (Conclusion) for your formal lab report are below:
Answer each of these questions in complete sentences. Use details from the background information and lab procedures to justify all of your answers. Make sure you answer each component of all questions.
1. For each of the 4 plates that you created, explain your results, what the correct results should have been and WHY, and any sources of error you, or other groups could have had with that plate. (You should have at least 2 sentences per plate)
2.Explain why it is difficult to get the bacteria to take in plasmid (include what we did in the lab to get the plasmid inside the bacteria) and why the bacteria may choose to not keep the plasmid.
3.What is the purpose of the ampicillin in this experiment?
4.Explain why the bacteria with the plasmid would have ditched the plasmid or died if they had not been isolated.
5.Conclusion: In a cohesive paragraph, describe how scientists created the recombinant DNA used in this lab (pGLO plasmid). Also describe & give examples of how scientists apply this recombinant DNA & transformation technique to scientific research (In your paragraph include why the gfp gene is a useful genetic tag).
**also make sure that you have a turnitin.com account and have added accelerated biology as a course: instructions are here**
