2. Review Recombinant DNA using Bacterial Plasmids Activity
3. Notes: Green Fluorescent Protein
H.W. Part I: Take out a new sheet of paper. Title the paper "Transformation Lab." Create a "Background Information" heading. Read the Transformation Lab Background Information (click on the name of the document to open). Then paraphrase (use your OWN words) 2-4 important bullet points from each paragraph under this heading.
Part II: Create a "Prelab Questions" heading. Read the Transformation Lab Procedure and then answer the following questions in complete sentences under this heading.
- What does it mean when a cell is competent?
- How do we make cells competent?
- What are two important sequences on a plasmid? Why are they important?
- Describe our gene of interest (What is the source? What protein does it code for?).
- How many tubes will you have? What goes in each tube?
- How many plates will you have? Draw 4 plates (circles). Under each plate describe what will go on each plate?
