2. Transformation Lab: the exciting results!!!! (absent today? Make sure you get your lab results from another member of your lab group. Also, on Monday I need to stamp your procedural flow chart and hypotheses)
H.W. Start typing your Transformation Lab. This is due on TUESDAY. Create the following headings and then place the requirements that are listed below after them:
- Title: Title the lab
- Purpose: State the purpose of the lab in YOUR OWN WORDS
- Background Information: Take the 10 bullet points that you wrote after reading the Transformation Lab Background Information and create 2 cohesive paragraphs. Your bullet points should already be in your own words, but if they are not, make sure that the 2 paragraphs are. You are paraphrasing : "a restatement of a text, passage, or work giving the meaning in another form."
- Prelab: Type the answers to your prelab questions.
- Hypotheses: Type your 4 scientifically "meaty" hypotheses
- Materials: Read the Transformation Lab Procedure and bullet point all materials that were used in the lab. Ex. 4 sterile inoculating loops, 4 pipettes, thermometer, beaker, ice, hot water bath etc.....
- Procedure: Type this heading. Under the heading type "see procedural flow chart on page ____." You will then attach your colorful procedural flow chart that you have already completed to your lab.
- Data: Type this heading. Under the heading type "see data on page ____." You will then attach your data drawings from today to your lab. For each plate you need to draw (in color) and describe your data (ex. how many colonies, no growth, lawn growth etc...). Each plate must also be labeled with what was on the plate (Ex. LB+, LB-, etc...)
- Analysis: Answer the following questions in complete, well thought out sentences.
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- On which plate(s) did you see transformed bacteria? Explain, in detail why the transformed bacteria were successful in this medium.
- Which plate(s) had lawn growth? Did the lawn growth also glow? Explain, in detail why this type of growth occurred.
- What allowed you to isolate the transformed cells (those that took up plasmid) from those that did not take up the plasmid?
- If you did not get any results (transformation did not occur), what factors could have affected your experiment?*** only for groups that did not have successful transformation
- In a cohesive paragraph, describe how scientists create recombinant DNA and transform bacteria. Also describe & give examples of how scientists apply this transformation technique to scientific research.
- Attach your stamped rough draft to the back of your formal lab report. Your rough draft should have a total of 2 stamps.
