3/14/14

1. Any final pGlo Transformation lab questions? (Recombinant DNA Review Video)
2. From cloning genes to cloning extinct animals??
3. Stem Cell and Cloning Web Assignment

H.W. Finish Stem Cell and Cloning Web Assignment
and Work on pGlo Transformation formal lab report 
DUE MONDAY NIGHT AT 11:59 PM TO TURNITIN.COM AND A HARD COPY IS DUE TO ME ON TUESDAY

BIOTECHNOLOGY QUIZ ON MONDAY

3/13/14

1. W.U. A Nobel for Illuminating Biology (article and questions)
2. Transformation Lab: the exciting results!!!!  (absent today?  Make sure you get your lab results from another member of your lab group.)

H.W.  Start typing your pGlo Transformation formal lab report 
DUE MONDAY NIGHT AT 11:59 PM TO TURNITIN.COM AND A HARD COPY IS DUE TO ME ON TUESDAY

Analysis Questions (Conclusion) for your formal lab report are below:

Answer each of these questions in complete sentences. Use details from the background information and lab procedures to justify all of your answers. Make sure you answer each component of all questions.

1. For each of the 4 plates that you created, explain your results, what the correct results should have been and WHY, and any sources of error you, or other groups could have had with that plate. (You should have at least 2 sentences per plate)
2.Explain why it is difficult to get the bacteria to take in plasmid (include what we did in the lab to get the plasmid inside the bacteria) and why the bacteria may choose to not keep the plasmid.
3.What is the purpose of the ampicillin in this experiment?
4.Explain why the bacteria with the plasmid would have ditched the plasmid or died if they had not been isolated.
5.Conclusion: In a cohesive paragraph, describe how scientists created the recombinant DNA used in this lab (pGLO plasmid). Also describe & give examples of how scientists apply this recombinant DNA & transformation technique to scientific research (In your paragraph include why the gfp gene is a useful genetic tag).

**also make sure that you have a turnitin.com account and have added accelerated biology as a course:  instructions are here**

3/12/14

1.  Transformation LAB
(Absent?  Or still confused about the lab?  Go to this virtual lab: http://www.phschool.com/science/biology_place/labbench/lab6/intro.html )

H.W. Finish your lab hypotheses (see below) and start typing your formal lab report
Transformation Lab Hypotheses: You will have a total of 4 hypotheses.
•For the E. Coli in each petri dish say if you expect growing AND glowing. These are the contents of your four plates:
a.LB/Amp+ (luria broth, ampicillin, plasmid) – this is an experimental plate
b.LB/Amp- (luria broth, ampicillin, no plasmid) – this is a negative control
c.LB+ (luria broth, no ampicillin, plasmid) – this is a positive control
d.LB- (luria broth, no ampicillin, no plasmid) – this is a positive control
•Use an if….then….because statement for each one of the plates above.
For example:
•If the petri dish contains _____________ (ex. Luria broth and Ampicillin) and the E.coli is given/not given (choose one) the plasmid, then the E.coli will ___________ (grow and glow, grow and not glow, not grow and not glow ect….) because ___________(insert something scientific here based on the background info)

3/11/14

1. Start drawing your procedural flow chart for the pGLO Transformation Lab
2. Review Transformation Lab
3. Why use antibiotic resistance?

H.W. Finish pGLO Transformation Lab procedural flow chart using these procedures (instructions are below):
• Read through the lab procedure for the transformation lab tomorrow.
• On a blank piece of white paper (unlined, printer paper), use pencil to draw pictures for each step of the procedure.
•Number the pictures and add captions that include all of the important, paraphrased information for each step.
•Make sure that you spend time making the drawing neat and accurate. This will go into a formal lab write-up for this lab.

And start typing your formal lab report

3/10/14

1. W.U. Biotech self quiz #1
2. Notes: Recombinant DNA, Transformation and antibiotic resistance as a form of bribery

H.W. pGLO Transformation Lab Background Information:  (Part I and II)
Part I: Complete the web activity below. 

Visit http://www.dnalc.org/resources/animations/index.html and access the two Transformation animations in the "biology animation library" section.  

As you step through the animations, answer the questions below:

Describe Cohen & Boyer's Experiment.
1. Include a description of pSC101 & pSC102
2. Which restriction enzyme was used? Identify its restriction/recognition sequence.
3. What is the role of DNA ligase?
4. Use the Transformation 2 animation to draw a picture of the process of making the bacterial cells competent. Include nicely drawn pictures and words!
5. Describe the two methods that Cohen & Boyer used to select for transformed bacteria. Include a picture that summarizes each method. (note: this is in the 1st Transformation animation)

Part II: Then, open this attached document and read through the background information for the lab we will complete on Wednesday/Thursday.  On your own paper, as you read, write down 3-4 important points (use bullet points, incomplete sentences are okay) per paragraph.  Additionally, redraw the bacterial cell from the background and label all of the parts as they are in the picture.